Figure 10.

Neurotoxic effect of D25–57 doppel peptide on primary cortical neurons from embryonic 17.5 d WT mice. Neurons were chronically exposed to the indicated concentrations of D25–57 peptide for 48 h at 37°C. Neuronal viability was monitored by using the cell proliferation reagent CCK-8 assay. The neurotoxicity of 160 μm P25–57 peptide was statistically significant after a 48 h incubation time compared with 40 and 80 μm and with the control scrambled peptide. Significant differences (p < 0.01) were tested using a two-way ANOVA Bonferroni's post hoc test.