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. 2013 Dec 4;33(49):19250–19261. doi: 10.1523/JNEUROSCI.2148-13.2013

Figure 2.

Figure 2.

Rotenone- and vincristine-induced neurite degeneration. A, Exposure of neurites to rotenone (1 μm) induces substantial degeneration at day 3 and nearly complete degeneration at day 6 after administration. Blockade of sodium channels with TTX (0.3 μm) and reverse NCX activity with KB-R7943 (0.5 μm) partially protects rotenone-treated neurites from degeneration. B, Across-treatment-group comparisons demonstrates that TTX provides significant protection from rotenone-induced degeneration at days 3 and 6, whereas KB-R7943 provides significant protection at day 3 after treatment only (*p < 0.05 compared with equivalent day of rotenone treatment only, n.s. = not significant; n = 7, 6, and 7 compartments for rotenone, rotenone + TTX, and rotenone+KB-R7943 conditions, respectively). Neurite lengths are normalized to pretreatment lengths (pre) for each individual neurite compartment at days 3 and 6, respectively. C, Exposure of neurites to vincristine induces substantial degeneration at day 3 after administration and increased degeneration at day 6. TTX and KB-R7943 treatment does not provide a protective effect on vincristine-induced neurite degeneration at day 3 or day 6 after administration. D, Across-treatment-group comparisons demonstrate that TTX and KB-R7943 do not provide significant protection from vincristine-induced neurite degeneration at days 3 and 6 (not significant, n.s.; compared with equivalent day for vincristine only; n = 7, 8, and 5 compartments for vincristine, vincristine + TTX, and vincristine + KB-R7943 conditions, respectively). Neurite lengths are normalized to pretreatment lengths (pre) for each individual neurite compartment at days 3 and 6, respectively. E, Neurites of DRG neurons from Nav1.8-Cre/tdTomato mice within peripheral culture chamber compartments exhibit sodium channel (green; top left) and NCX2 (green; top right) immunoreactivity. F, Control neurites treated with TTX or KB-R7943 exhibit continued growth at days 3 and 6 after administration, demonstrating that blockade of sodium channels with TTX or of reverse NCX with KB-R7943 does not alter the temporal distribution of neurite lengths compared with equivalent day of control; (not significant, n.s.; n = 4 compartments each for control, control + TTX, and control + KB-R7943 conditions). Data are presented as mean ± SEM.