Skip to main content
. 2013 Dec 4;33(49):19284–19294. doi: 10.1523/JNEUROSCI.2542-13.2013

Figure 3.

Figure 3.

pBriAβeGPI and pBriAβe undergo proteolytic processing, but, although tg mice do not show plaques, both promote Aβ fibrillization. A, Western blot analysis of expression levels of HEK AβeGPI (lane 1) and HEK Aβe (lane 2) cells (cell hom.), compared with brain extracts (brain hom.) from 2-month-old tg Aβe (lane 3, 4), AβeGPI (lane 5, 6), and APP23 mice (lane 7, 8) with Aβ antibody 6E10. B, Western blot analysis of brain extracts from 24-month-old tg Aβe (lane 1, 2), AβeGPI (lane 3, 4), and APP23 (lane 5, 6) mice. C, Quantification of Western blots comparing the total expression levels of Aβe and AβeGPI to Aβ (100%) as a percentage in 2- and 24-month-old tg Aβe, AβeGPI, and APP23 mice. Aβe and AβeGPI are significantly lower expressed than Aβ in 2-month-old tg APP23 mice (100%) and stay merely unchanged at 24 month in contrast to 24-month-old tg APP23 mice (∼1200%). There are significant differences between the groups, as indicated (n = 2 mice per group for all 2-month-old tg mice, and n = 3 mice per group in all 24-month-old tg mice; *p < 0.05, ***p < 0.001; ns, not significant, one-way ANOVA, Bonferroni's post hoc test for multiple comparisons). D, Histological analysis of 24-month-old tg Aβe and AβeGPI mice. Coronal sections of respective tg lines through the frontal and entorhinal cortex stained with Aβ antibody CN3 and Congo red. Amyloid deposition was observed in neither tg Aβe nor AβeGPI mice. APP23 mice at the age of 16 months served as positive control of amyloid deposition. Scale bar, 500 μm. E, Fibrillization of recombinant Aβ1–40 was monitored by incorporation of Thioflavin T. Kinetics are displayed as time versus relative fluorescence. Data points represent the mean and SE of eight technical replicates of a representative individual mouse of the indicated genotype. Brain homogenates containing fibrillization seeds reduced the lag phase until there is a steep fluorescence increase to reach plateau. F, Comparison of lag times extracted from E. At the age of 24 months, APP23 containing Aβ plaques as well as AβeGPI had reduced lag times compared with age-matched WT mice. Significant differences are only found between the groups indicated (*p < 0.05, one-way ANOVA, Bonferroni's post hoc test for multiple comparisons; 2-month-old APP23 mice, n = 4; 2-month-old WT mice, n = 3; 2-month-old AβeGPI mice, n = 4; 2-month-old Aβe mice, n = 4; 24-month-old APP23 mice, n = 2; 24-month-old WT mice, n = 2; 24-month-old AβeGPI mice, n = 4; 24-month-old Aβe mice, n = 6).