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. 2019 Mar 6;125(13):2222–2232. doi: 10.1002/cncr.32033

Figure 4.

Figure 4

Heptamethine carbocyanine fluorescent dye–cisplatin conjugate (DZ‐CIS)–induced caspase cascade activation. (A) Namalwa cells treated with DZ‐CIS were assayed for the involvement of caspases with a CaspaseGlo 3/7 luminescent assay 24 hours after treatment. Results are presented with the mean ± standard deviation of quadruple measurements (* P < .05, ** P < .01, *** P < .0001). (B) Whole cell lysates of the treated cells were subjected to western blotting to detect activation of the caspase cascade. (C) DZ‐CIS–treated Namalwa cells were examined for protein level and protein modification changes by western blotting. DZ‐CIS treatment showed decreased expression of c‐myc and induced Max protein cleavage and increased p27 protein expression, suggesting growth arrest of treated cells. DZ‐CIS treatment caused cleavage of the ROCK1 protein. c‐Jun, STAT3, and Src protein phosphorylation was suppressed. Paradoxically, DZ‐CIS caused increased phosphorylation of AKT, suggesting its activation.