Figure 5.
The heptamethine carbocyanine fluorescent dye–cisplatin conjugate (DZ‐CIS) inhibited xenograft tumor growth. (A) NCrnu/nu mice (top, n = 5 nude mice bearing 8‐10 Namalwa tumors per treatment group) and NOD Scid mice (bottom, n = 5 Scid mice bearing 16 Namalwa tumors per treatment group) were treated intraperitoneally with either 5 (NOD Scid) or 10 (NCr nude) mg/kg DZ‐CIS twice a week, from day 4 and day 1, respectively, resulting in significantly decreased tumor volume compared with CIS or vehicle treatment groups (P < .05). (B) CIS and DZ‐CIS–treated xenograft tumors were analyzed by immunohistochemistry for cell proliferation, survival, and death biomarker protein expression. (C) Mice were evaluated for renal and hepatic injuries with immunohistochemistry for cell death markers.