Figure 7.

Cx43 and Cx47 expression and phosphorylation in Cx43floxG138R/Cx30KO Nestin–Cre mice. Immunostainings for Cx43G138R (A; green) and Cx47 (B; red) were performed on 25 μm brain slices of 50-d-old Cx43^ODDD mice. Both connexins are expressed at the cingulum of Cx43^ODDD mice. Immunoblot analysis (C) shows that Cx43G138R is not phosphorylated. Similar to WT (Fig. 3), Cx47 protein is phosphorylated in Cx43^ODDD mice. Fifty micrograms of protein per lane were loaded onto the SDS-gel. Tubulin was used as loading control. Scale bar, 100 μm.