Figure 11.

p62 overexpression does not affect AR expression. A, Full-length wild-type (24Q) or mutant (97Q) AR and HA-tagged p62 (pCAGGS–p62–HA) were cotransfected into Neuro2A cells for 48 h. B, Full-length wild-type (24Q) or mutant (97Q) AR and FLAG-tagged p62 (pIRESpuro3–3XFLAG–p62) were cotransfected into NSC34 cells for 48 h. All of the cells were cultured in DMEM/10% FCS at 37°C and with 5% CO₂. Immunoblots revealed that the expression of AR was similar in the presence or absence of HA–p62 or FLAG–p62.