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. 2013 May 1;33(18):7710–7727. doi: 10.1523/JNEUROSCI.3021-12.2013

Figure 3.

Figure 3.

Depletion of p62 promotes the accumulation of AR. A, PC12 cells expressing wild-type (10Q) and mutant (112Q) AR were transfected with either control or p62 siRNA. The cells were treated with DHT for 3 or 7 d. B, Neuro2A cells transfected with wild-type (24Q) and mutant (97Q) AR were cotransfected with control or p62 siRNA. C, Pulse-chase analysis of two forms of AR in PC12 cells. Data from one representative experiment for wild-type and mutant AR. D, Pulse-chase assessment of half-life of the wild-type (left) and mutant AR (right). The percentages of wild-type (10Q) and mutant (112Q) remaining in the presence (●) and absence (○) of p62 siRNA are indicated. E, Real-time RT-PCR for wild-type (10Q) and mutant (112Q) AR mRNA normalized to GAPDH levels in PC12 cells. F, Real-time RT-PCR for wild-type (24Q) and mutant (97Q) AR mRNA normalized to GAPDH levels in Neuro2A cells. These experiments were repeated in five sets of cells, and equivalent results were obtained. All of the values are expressed as the means ± SEM (n = 5). *p < 0.05; **p < 0.01.