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. 2013 May 1;33(18):7710–7727. doi: 10.1523/JNEUROSCI.3021-12.2013

Figure 4.

Figure 4.

Depletion of p62 impairs behavioral and visible phenotypes in male AR–97Q mice. A–D, p62 immunohistochemistry in the spinal anterior horn (A, B) and skeletal muscle (C, D) of 25-week-old AR–97Q/p62+/+ (A, C) and 13-week-old AR–97Q/p62−/− (B, D) mice counterstained with Mayer's hematoxylin. p62 immunoreactivity was localized to the nuclei and cytoplasm, with NIs (arrow) in the anterior horn cells and skeletal muscle. Scale bars, 20 μm. E, Western blotting analysis of p62 expression in the total spinal cord and muscle protein lysates from the indicated mice immunolabeled with antibodies against p62. F–J, Rotarod task (F), cage activity (G), grip strength (H), body weight (I), and survival rate (J) of the AR–97Q/p62+/+(●; n = 20), AR–97Q/p62+/−(▾; n = 28), and AR–97Q/p62−/− (■; n = 20) mice. Although none of the parameters tested at 15 weeks revealed significant differences between AR–97Q/p62+/− and AR–97Q/p62+/+ mice, the AR–97Q/p62+/− mice performed more poorly than the AR–97Q/p62+/+ mice in all of the parameters. K, Footprints of representative 13-week-old AR–97Q/p62+/+ and AR–97Q/p62−/− mice. The front paws are indicated in blue, and the hindpaws are indicated in red. Values are expressed as the means ± SEM. *p < 0.05.