Skip to main content
. 2013 May 1;33(18):7710–7727. doi: 10.1523/JNEUROSCI.3021-12.2013

Figure 5.

Figure 5.

Depletion of p62 induces the accumulation of AR in an SBMA mouse model. A–F, PolyQ immunohistochemistry (1C2) in the spinal anterior horn (A–C) and muscle (D–F) of 13-week-old AR–97Q/p62+/+, AR–97Q/p62+/−, and AR–97Q/p62−/− mice. Scale bars, 50 μm. G, H, Quantitative assessment of 1C2 staining in the spinal ventral horn (G) and muscle (H). Bars represent the density of 1C2-positive cells in AR–97Q/p62+/+, AR–97Q/p62+/−, and AR–97Q/p62−/− mice. The results are expressed as the means ± SEM (n = 6). I, J, Western blotting analysis of the total tissue homogenates from the spinal cord (I) and muscle (J) of AR–24Q and AR–97Q mice (13 weeks of age, n = 6), probed with anti-AR. Values are expressed as the means ± SEM (n = 6). K, L, Immunohistochemical staining with anti-GFAP antibody in the spinal anterior horn. Scale bars, 30 μm. M, Western blotting analysis of the total tissue homogenates from the spinal cord of AR–97Q mice (13 weeks of age, n = 6) probed with anti-GFAP. N, O, Hematoxylin and eosin staining of the muscles. P, The gastrocnemius muscles from AR–97Q/p62+/+ and AR–97Q/p62−/− mice were dissected and weighed. Q, Western blotting analysis of the total tissue homogenates from the spinal cord and muscle of AR–24Q and AR–97Q mice (13 weeks of age) probed with anti-ubiquitin (Ub). Scale bars, 50 μm. *p < 0.05; **p < 0.01; ***p < 0.001.