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. 2013 May 1;33(18):7710–7727. doi: 10.1523/JNEUROSCI.3021-12.2013

Figure 7.

Figure 7.

p62 promotes inclusion body formation of ARs in male AR–97Q mice. A, Immunohistochemical staining with 1C2 antibody revealed DNS (arrowhead) in AR–97Q/p62(+/+) mice and many NIs (arrow) in the AR–97Q/p62(tg/+) mice in the spinal anterior horn and skeletal muscle at 25 weeks of age. The region of interest for the DNS is defined as the ROI (inset; open square). Scale bars, 30 μm. B, The ratio of the number of cells with NIs to the total number of IC2-positive cells. C, The ratio of the number of cells with DNS to the total number of IC2-positive cells. D, The R.S.I. of the DNS was significantly reduced in cells with NIs compared with those without NIs in spinal anterior horn neurons and skeletal muscle. E, F, Double-immunofluorescence staining with 1C2 (red) and anti-HA (green) antibodies in the spinal anterior horn (E) and skeletal muscle (F) of AR–97Q/p62(tg/+) mice revealed the complete colocalization of HA-tagged p62 and mutant AR in NIs (arrow), but no colocalization was observed in the DNS (arrowhead). Scale bars, 20 μm. G, H, p62 immunohistochemistry in the spinal anterior horn neurons (G) and skeletal muscle (H) of AR–97Q/p62(tg/+) mice. Arrows indicate NIs. Scale bars: G, 30 μm; H, 50 μm. Values are expressed as the means ± SEM (n = 6). I, 1C2 (red) and anti-HA (green) double immunofluorescence in the spinal cord of 25-week-old AR–97Q/p62(tg/+) mice. *p < 0.01; **p < 0.001.