The number of neurons that responded to ipsilateral limb stimulation increased after unilateral SSC stroke in contralateral SSC. A, Percentage of neurons responding to each limb stimulation. The percentage significantly increased after the stroke, particularly in the iFL- and iHL-stimulated groups. B, Percentage of neurons responding to single-limb or multiple-limb stimulation (2, 3, or 4). The percentage in each group was calculated by dividing the number of neurons responding to one, two, three, or four limb stimulation by the number of all neurons showing a positive response to any limb stimulation. Inset, The percentage of neurons responding to both ipsilateral and contralateral limb stimulations (cFL + iFL or cHL + iHL). C, Peak amplitude (%ΔF/F0) of the change in intracellular calcium transients after limb stimulation. No significant changes were observed, except when cHL was stimulated in the 2 week group, which showed a weak increase. In all the graphs, data are mean ± SEM. *p < 0.05, compared with Sham (Bonferroni test). **p < 0.01, compared with Sham (Bonferroni test). n.s. indicates not significant.