Figure 3.

The amplitude of calcium transients of astrocytes that responded to ipsilateral limb stimulation increased after the unilateral SSC stroke in the contralateral SSC. A, Percentage of astrocytes responding to each limb stimulation. The percentage particularly increased in the iFL- and iHL-stimulated groups after the stroke. B, Percentage of astrocytes responding to single-, or multiple-limb stimulation (2, 3, or 4). The percentage in each group was calculated by dividing the number of astrocytes responding to one-, two-, three-, or four-limb stimulation by the number of all astrocytes showing a positive response to any limb stimulation. Inset, The percentage of astrocytes responding to both ipsilateral and contralateral limb stimulations (cFL + iFL or cHL + iHL). The number of astrocytes receiving information on only single-limb stimulation markedly decreased after the stroke. C, Peak amplitude (%ΔF/F₀) of the change in intracellular calcium transients after limb stimulation. No significant changes were observed, except when cHL was stimulated in the 2 week group, which showed a weak increase. Interestingly, the amplitude significantly increased particularly in the iFL- and iHL-stimulated in the 1 week group. *p < 0.05, compared with Sham (Bonferroni test). **p < 0.01, compared with Sham (Bonferroni test). n.s. indicates not significant.