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. 2019 Apr 4;49(7):1001–1014. doi: 10.1002/eji.201847971

Figure 4.

Figure 4

Effects of HMOS‐conditioned human moDCs on the induction of Tregs and IL‐10 production by T‐cell. (A) Representative plots of CD4+CD25+Foxp3+ Tregs induced by control DCs treated with medium, LPS, HMOS (5 mg/mL), and HMOS (5 mg/mL) +LPS matured DCs. Gating strategy is shown in Supporting Information Fig. 4. (B) Percentage of CD25+Foxp3+ Tregs from CD4+ T‐cell 6–7 days’ post MLR assay. Stimulated DCs were collected at 24 h and co‐cultured with fresh isolated naïve CD4+ T‐cell in a 1:10 ratio. After 6–7 days of co‐culture, T‐cell was harvested and analyzed for the expression of CD4‐PerCP‐Cy5.5, CD25‐FITC and Foxp3‐PE‐Cy7 by flow cytometry. (C) IL‐10 secretion in the supernatant of MLR. Supernatant was collected for IL‐10 measurement by ELISA assay. Results are presented as mean ± SEM (4 independent experiments (2‐3 donors/experiment) for panel B, (3 independent experiments (1‐2 donors/experiment) for panel C), *p < 0.05, **p < 0.01, ****p < 0.0001, paired Student's t‐test.