Figure 1.

AngII type1 receptor (AT1) mRNA is expressed in mouse taste bud cells. A, RT-PCR amplification of AT1, AT2, Trpm5, Pkd2L1, and β-actin mRNAs from FP, VP, and tongue epithelium devoid of taste cells (ET). RT+ and RT− conditions are, respectively, with and without reverse transcriptase. M, 100 bp marker ladder. B, ISH detection of AT1, T1r3, Trpm5, and Pkd2L1 in FP and VP of B6 mice. The sense probe for AT1 serves as a negative control. Dotted lines indicate the outline of taste buds. Scale bar, 50 μm.