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ChR2 transfection. A, Light microscopic image of primary neuronal culture at DIV9 on a 60 electrode MEA transfected with plasmid DNA encoding Channel Rhodopsin-2 fused to Yellow Fluorescent Protein (ChR2-YFP). B, Image taken using a 4× objective and 510 nm excitation light to visualize ChR2-YFP expression. Cell count indicates approximately 2% transfection efficiency.
