Figure 4.

PKRi decreases phosphorylation of PKR (Thr451) and eIF2α (Ser51) in hippocampal slices. A, Hippocampal slices were incubated with PKRi or vehicle containing 0.5% DMSO for 0.5, 1, 2, or 3 h. Protein phosphorylation is expressed as the ratio between antiphosphospecific antibody and antiprotein-directed antibody, normalized to vehicle values. Left panels of the bar graphs are representative immunoblots. Phosphorylation of PKR on Thr451 diminished 0.5–3 h after application of 50 μm PKRi relative to the level in the vehicle-exposed group (n ≥ 4). *p < 0.05. **p < 0.01. eIF2α phosphorylation on Ser51 also decreased 2 h after PKRi application (n ≥ 4). *p < 0.05. B, PKRi does not affect other important pathways of translational control. ERK2 and S6K1 phosphorylation is not changed 0.5–3 h after application of 50 μm PKRi relative to vehicle-exposed group (n ≥ 4). p > 0.05. C, PKRi does not affect the levels of β-actin or β-tubulin compared with vehicle exposure. Data are mean ± SEM.