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Cilia of neurons overexpressing 5HT6:EGFP do not contain detectable levels of SSTR3. A, Control P14 neocortex stained with antibodies against ACIII (green) and SSTR3 (red). The majority of ACIII+ cilia are also SSTR3+ (arrows). Scale bar, 10 μm. B, Cilia synthesized by neurons expressing 5HT6:EGFP (arrows) are not correspondingly SSTR3+. Scale bar, 10 μm. C, The percentage of ACIII+ control (n = 1056) or 5HT6:EGFP+ (n = 688) cilia that are also SSTR3+.
