Table 1.
Primer name | Gene target | Sequence (5′–3′) | Product length (bp) |
---|---|---|---|
β-actin-F | β-actin | aaatcgtgcgtgacatcaaa | 178 |
β-actin-R | aaggaaggctggaaaagagc | ||
mProm2305F | Prominin-1 universal | tgaagaagatccttgcctc | 210 |
mProm2515R | tccgcaacatagccacac | ||
Prom2–629F | Prominin-2 | cacacacatagtcagacgg | 206 |
Prom2–835R | gtacactgtgctcttgag | ||
mProm56F | Prominin-1.s1 | ccgagacccggaggagagc | 477 |
mProm533R | tagagggcaatctccttgg | ||
mProm56F | Prominin-1.s2 | ccgagacccggaggagagc | 438 |
mProm494R | gccaagactatgatttctgg | ||
mProm2557F | Prominin-1.s3/4/5 or s6 | tctgtgtggctatgttgcgg | 230 or 467 |
mProm2728R | catgcatgttcaccagacac | ||
mProm2236F | Prominin-1.s8 | gaaatttgtgagggtgagg | 418 |
mProm2654R | cctctgaatccatcctgcg |
F, forward; R, reverse; bp, base pair.