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. 2019 Jun 13;36(5):237–247. doi: 10.1002/yea.3390

Figure 2.

Figure 2

Method for downregulation of target genes. (a) Two heterologous genes AGO1 and DCR1 from Naumovozyma castellii were overexpressed in a yeast strain already expressing Cas9 and CFP‐YFP‐RFP genes. (b) Schematic illustration of USER assembly of the downregulation cassette. (c) Fluorescence images of yeast colonies expressing either individual fluorescent proteins, three fluorescent proteins (XFP), or expressing XFPs, and a downregulation construct for CFP/YFP [Colour figure can be viewed at wileyonlinelibrary.com]