ChAT labeling in monkey spinal cord. A, ChAT labeling in a transverse section (50-μm-thick) of monkey lumbar spinal cord. A strong cholinergic labeling of motoneurons is observed in the ventral part of the cord, some neurons are labeled around the central canal and deep dorsal horn, and a cholinergic plexus is present in the superficial dorsal horn. What is observed in the upper right edge of lamina I (next to the dorsal root entry zone) is a nonspecific deposit of DAB reaction product. B, ChAT labeling in a transverse section (30-μm-thick) of monkey cervical spinal cord. C, High magnification of ChAT-positive neurons in transverse sections (30-μm-thick) of monkey lumbar (C1–C3) or cervical (C4,C5) spinal cord. Scale bars: A, B, 500 μm; C, 50 μm.