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. 2019 Jul 3;10:834. doi: 10.3389/fphys.2019.00834

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Copyright © 2019 Rougier, Essers, Gillet, Guichard, Sonntag, Shmerling and Abriel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the detubulation procedure. (A) Confocal images showing detubulation mediated by formamide treatment on living cardiomyocytes. (B) Effect of detubulation on cell capacitance (pF). (C,D) functional consequences of detubulation on barium currents (I_Ba) carried by calcium channels. (^∗∗p < 0.01, ^∗∗∗p < 0.001). The number of cells is indicated in parentheses.