| Antigen-specific B cell sorts | |||
|---|---|---|---|
| Marker | Fluorochrome | Company | Clone |
| Env probe-biotin | Alexa Fluor 647 | Invitrogen | |
| Env probe-biotin | Brilliant Violet 421 | BioLegend | |
| Viability | efluor780 | Thermo Fisher | |
| CD4 | APC efluor780 | Thermo Fisher | SK3 |
| CD8a | APC efluor780 | Thermo Fisher | RPA-T8 |
| CD16 | APC efluor780 | Thermo Fisher | ebioCD16 |
| CD20 | Alexa Fluor 488 | BioLegend | 2H7 |
| IgG | PE-Cy7 | BD Biosciences | G18-145 |
| IgM | PerCP-Cy5.5 | BD Biosciences | G20-127 |
| CD38 | PE | NHP Reagents | OKT |
| CD71 | PE-CF594 | BD Biosciences (custom) | L01.1 |
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