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. 2013 Apr 24;33(17):7488–7500. doi: 10.1523/JNEUROSCI.3849-12.2013

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Figure 8. — Analysis of tetraploidy in SPNs. Cell nuclei isolated from the cerebral cortex of P0 mice were coimmunostained with anti-Sox5 and anti-CTIP2 antibodies, and subjected to flow cytometric analysis after labeling with PI. DNA content (Propidium Iodide-A) plotted against CTIP2 signal intensity (CTIP2 FITC-A) was evaluated in the Sox5-positive population. The horizontal line represents the threshold for CTIP2-specific staining. The Sox5-positive/CTIP2-negative population (i.e., SPNs) mainly contains diploid neurons.