Fig 5. Decreased intestinal inflammation in mice deficient in iCD8α cells.

Rag-2^-/- and E8_I^-/-Rag-2^-/- mice were treated with anti-CD40 and monitored for 7 days for weight change (a). (b) At the endpoint, colons were harvested for pathological analysis. (c) Osteopontin mRNA expression from the colons of anti-CD40 treated Rag-2^-/- and E8_I^-/-Rag-2^-/- mice at the indicated time points. Data is representative of at least two independent experiments (n = 6 to 8). (d) E8_I^-/-Rag-2^-/- mice were treated with recombinant osteopontin or PBS at day -2, -1 and 1 before and after disease induction with 70μg of anti-CD40 antibodies, and their weights monitored for 7 days. (e) At the endpoint, colons were harvested for pathological analysis. Data is representative of at least 3 independent experiments (n = 6 to7). (f) Total NKp46⁺NK1.1⁺ IEL in the indicated mice after day 7 of anti-CD40 treatment. Data is representative of at least 3 independent experiments (n = 6 to7). Each symbol represents and individual mouse. *p<0.05, **p<0.01, ***p<0.01 using two-way ANOVA (a, d), unpaired two-tailed Student’s T test (b, c) or one-way ANOVA (e).