Table 1. Assay validation precision and accuracy.

Biomarker	Nominal Concentration (pg/mL)	Mean Measured Concentration (pg/mL)	% CV	% Theoretical
IL-6	2.45 (LLOQ)	2.58	1.0	105.3
	7.72	7.38	3.3	95.6
	54.50	54.30	3.2	99.6
	265	263	0.9	99.2
	444 (ULOQ)	476	2.2	107.2
CRP	69.2 (LLOQ)	67.7	1.4	97.8
	6440	6073	0.5	94.3
	1940	17907	1.2	92.3
	68000	71871	0.2	105.7
	108000 (ULOQ)	131880	4.0	122.1
TNFα	0.79 (LLOQ)	0.87	3.0	113.5
	2.17	2.08	4.2	95.9
	16.4	14.8	1.4	90.2
	95.5	85.7	2.9	89.7
	211 (ULOQ)	211	2.5	100.0
MCP-1	8.28 (LLOQ)	8.67	1.8	104.8
	11.9	11.0	9.8	92.4
	69.2	63.5	5.5	91.8
	438	430	3.2	98.2
	1060 (ULOQ)	1080	4.3	99.5
MIP-1β	17.5 (LLOQ)	24.5	7.8	90.4
	142	132.0	4.6	93.0
	560	544.5	1.9	97.2
	776	745	1.3	96.0
	2240 (ULOQ)	2330	0.4	104.0
Eotaxin	56.8 (LLOQ)	57.4	4.8	101.0
	180	168.0	2.3	93.3
	553	520	4.5	94
	1480	1410	2.2	95.3
	3640 (ULOQ)	3775	8.6	103.7
VCAM-1	90.8 (LLOQ)	89.1	1.0	98.1
	1690	1536	0.6	90.9
	5110	4786	0.9	93.7
	17900	16826	1.8	94.1
	28400 (ULOQ)	28201	5.1	99.3

CV; coefficient of variance; LLOQ; lower limit of quantification, ULOQ; upper limit of quantification