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(A) Anti-GFP co-immunoprecipitation of GFP-PGRN or individual GFP-granulins and GBA-myc overexpressed in HEK293T cells shows binding primarily to Grn F, with weaker binding to Grn A and other Grn peptides. (B) Anti-GFP co-immunoprecipitation of GFP-PGRN or individual GFP-granulins and GBA-myc from conditioned medium of transfected cells shows weaker binding to all the granulins except Grn G.
