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Western blot assay for expression of HIF-1α showing results presented to confirm equal loading of the protein samples. Relative densities of HIF-1α were normalized by the respective amounts of Ponceau S red in 30-d-old male rat pups of dams fed an NP or an LP diet during pregnancy and lactation. HIF-1α, hypoxia-inducible factor 1α LP, offspring of experimental rat dams fed a low-protein diet (8% protein); NP, offspring of control rat dams fed a normoproteic diet (17% protein).
