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. 2013 Aug 7;33(32):12997–13009. doi: 10.1523/JNEUROSCI.5284-12.2013

Figure 6.

Figure 6.

In culture, actin-GFP-labeled cortical/hippocampal neurons coexpress presynaptic and postsynaptic markers. A, Cortical/hippocampal neurons cotransfected with Q69 huntingtin + actin-GFP express PSD-95 in most of the dendritic spines, suggesting that actin-GFP labeled protrusions in these neurons are true spines. B, Q17 huntingtin + actin-GFP overexpressing neurons showing PSD-95 immunopositive punctae in most spines. C, D, Synaptophysin (a presynaptic protein marker)-positive dots were found adjacent to dendritic spines in cortical/hippocampal neurons overexpressing Q69 and Q17 huntingtin. White arrows indicate synaptophysin or PSD-95 immunoreactivity close to or in the dendritic spines. The color of the text (Q-69 huntingtin + actin-GFP, Q17 huntingtin + actin-GFP, PSD-95, synaptophysin) represents the color of the antigen in the corresponding merged section (right). Scale bar, 5 μm.