Figure 2.
Structure of Goofy protein. A, Hydrophobicity plot of mouse Goofy protein. Two highly hydrophobic regions are present: one at the N terminus and the other around amino acid number 400, which potentially serve as a signal peptide (SP) and a transmembrane segment (TM), respectively. B, Amino acid sequence of mouse Goofy protein. Red underline, Signal peptide; blue underline, transmembrane segment; green double underline, canonical SH3-binding motif; black triangles, potential Asn-linked glycosylation sites. Ser, Thr, and Pro residues are highlighted in blue, green, and red letters, respectively. C, A schematic diagram depicting the domain structure of Goofy protein. Goofy consists of an N-terminal signal peptide (SP, red), a Ser/Thr/Pro-rich extracellular region (yellow) with three potential glycosylation sites (black triangles), a transmembrane segment (TM, blue), and a Pro-rich cytoplasmic region (green).