Figure 1.
Arctigenin reduced Aβ production with BACE1 translation inhibition in HEK293-APPswe cells. HEK293-APPswe cells were cultured with 0.1% DMSO (as a control) or different concentrations of arctigenin (ATG) for 24 h. A, Chemical structure of arctigenin. B, ELISA assay of arctigenin-decreased Aβ40. C, ELISA assay of arctigenin-reduced sAPPβ. D, Arctigenin inhibited BACE1 activity in cells. E, FRET-based assay demonstrated that arctigenin failed to inhibit BACE1 activity at protein level. F, Western blot assay indicated that arctigenin decreased BACE1 protein level and phosphorylation of eIF2α or PERK. G, RT-PCR assay indicated that arctigenin had no effect on BACE1 transcription. The results shown are representative of three independent experiments. **p < 0.01, ***p < 0.001, n = 3.