Figure 7.

Arctigenin decreased Aβ content and inhibited BACE1 translation in APP/PS1 AD mice. For Aβ analysis, the hippocampi were lysed in 5 m guanidine hydrochloride, followed by ELISA assay against Aβ₄₀ and Aβ₄₂, n = 10. A, Arctigenin decreased Aβ₄₀ or Aβ₄₂ content in hippocampus. B, Arctigenin decreased Aβ₄₀ or Aβ₄₂ content in cortex. The cortices were lysed in RIPA buffer for Western blot assay, n = 4. C, Arctigenin decreased BACE1 protein level and eIF2α or PERK phosphorylation. D, Densitometry analysis of C. E, RT-PCR assay indicated that arctigenin had no effect on BACE1 transcription in mice. For RT-PCR assay, BACE1 mRNA levels of the cortices were investigated, n = 4. T-V, Transgenic mice administrated with vehicle; NT-V, nontransgenic mice administrated with vehicle; T-ATG3, transgenic mice administrated with 3 mg/kg/d arctigenin. Values are means ± SE, *p < 0.05, **p < 0.01, ***p < 0.001, compared with T-V.