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. 2019 Jul 10;14(7):e0219210. doi: 10.1371/journal.pone.0219210

Fig 4. Predicted structures at exon-intron junctions that contain mutations and experimental results with minigenes at exon-intron junctions.

Fig 4

Shown are structures containing mutations that affect the wild type structure. Mutations are denoted in green. Splice sites are denoted with red arrowheads. (a) to (c) Extended structures of hairpins at the exon 10-intron 10 junction containing S305N, G(+3)A, and C(+14)T mutations, respectively. (d) and (e) Wild type and G(−10)T mutant hairpin structures, respectively, at the intron 9-exon 10 junction. (f) to (i) Minimal wild type and mutant S305N, G(+3)A, and C(+14)T hairpin structures, respectively, at the exon 10-intron 10 junction. (j) Effect of mutations at the 5′ and 3′ splice sites of exon 10 on luciferase activity in transfected HeLa cells. (k) Effect of mutations at the 5′ and 3′ splice sites of exon 10 on 3R and 4R tau mRNA levels in HeLa cells as measured by RT-qPCR.