Fig 1. Comparing methodology of original paper and present study and Abeta expressing C. elegans transgenic strains.

a) The model expresses human Abeta that results in alteration phenotypes (please refer to Material and Methods section), b) C. elegans strains used by Hassan and colleagues, c) Experimental design and methodology by Hassan and colleagues. Briefly, synchronized eggs of transgenic worms were grown at 16˚C for 36 h and shifted to 25˚C to activate the smg-1 gene. Worm samples were collected at 4 hours intervals from T0 (temperature shift time) to T20 (before worm paralysis), and analysis including microarray, PCR, gene ontology, and RNA interference were performed in the original study. d) Pipelines used in this study to analyze publicly available datasets GSE65851, and datasets for incipient AD including GSE12685 and GSE28146.