Table 3. OPLS analysis of metabolites with variable importance in projection for HIGA mice.

A. 12 weeks
Positive predicting variable		Negative predicting variable
Metabolites	VIP	Metabolites	VIP
Glucuronic acid-5TMS(2)	0.1504 ± 0.0226	Tyrosine-3TMS	-0.1496 ± 0.0611
Uracil-2TMS	0.1498 ± 0.0451	Coniferyl aldehyde-meto-TMS	-0.1204 ± 0.0816
Mannose-meto-5TMS(1)	0.1393 ± 0.0300	Tryptophan-3TMS	-0.0781 ± 0.0668
Allose-meto-5TMS(1)	0.1389 ± 0.0310	Aspartic acid-3TMS	-0.0769 ± 0.0709
Psicose-5TMS(1)	0.1324 ± 0.0482	2-Hydroxybutyric acid-2TMS	-0.0682 ± 0.0573
Myristic acid-TMS	0.1280 ± 0.0334	Serine-3TMS	-0.0544 ± 0.0511
B. 22 weeks
Positive predicting variable		Negative predicting variable
Metabolites	VIP	Metabolites	VIP
Glucuronic acid-5TMS(2)	0.1488 ± 0.0443	Tyrosine-3TMS	-0.1161 ± 0.1004
Xylitol-5TMS	0.1350 ± 0.0407	Aspartic acid-3TMS	-0.1057 ± 0.0480
Xanthine-3TMS	0.1350 ± 0.0377	Coniferyl aldehyde-meto-TMS(1)	-0.1032 ± 0.0810
Inosine-4TMS	0.1337 ± 0.0575	Threonine-3TMS	-0.0940 ± 0.0882
Threonic acid-4TMS	0.1326 ± 0.0420	O-Acetylserine-2TMS	-0.0877 ± 0.0874
Myristic acid-TMS	0.1303 ± 0.0521	Glycine-3TMS	-0.0868 ± 0.0786
C. 32 weeks
Positive predicting variable		Negative predicting variable
Metabolites	VIP	Metabolites	VIP
Glucuronic acid-5TMS(2)	0.1236 ± 0.0564	Phosphoenolpyruvic acid-3TMS	-0.1229 ± 0.0519
Alanine-2TMS	0.1015 ± 0.0440	Hypotaurine-3TMS	-0.1206 ± 0.0483
Leucine-2TMS	0.0996 ± 0.0420	Glycerol 3-phosphate-4TMS	-0.1185 ± 0.0298
Inositol phosphate-7TMS	0.0994 ± 0.0335	O-Phosphoethanolamine-4TMS	-0.1165 ± 0.0346
Xylitol-5TMS	0.0982 ± 0.0467	Eicosapentaenoic acid-TMS	-0.1150 ± 0.0355
Myristic acid-TMS	0.0940 ± 0.0299	Oxalic acid-2TMS	-0.1137 ± 0.0318

Data are the mean ± SEM. VIP means variable importance in projection, which was calculated in OPLS analyses using SIMCA.