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. 2019 Jun 30;24(2):123–128. doi: 10.15430/JCP.2019.24.2.123

Figure 2.

Figure 2

Effect of esculetin on H2O2-induced matrix metalloproteinase (MMP)-1 expression and activation. The mRNA (A) and protein levels of MMP-1 (B) were detected by reverse transcription (RT)-PCR and Western blotting (WB), respectively. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and actin were used as loading controls. (C) MMP-1 activity was examined using a Human Active MMP-1 Fluorescent Assay Kit. aP < 0.05 vs. control cells, bP < 0.05 vs. H2O2-treated cells.