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Effect of esculetin on H2O2-induced mitogen-activated protein kinases and intracellular Ca2+ levels. (A) Protein expression levels of phospho-MEK1 and phospho-ERK1/2 were detected by Western blotting (WB). (B) Expression levels of phospho-SEK1 and phospho-JNK1/2 were determined by WB. (C) Fluorescence indicating the Ca2+ level was captured by confocal microscopy using Flou-4-AM staining (×100).
