Table 1.
Primer sequences for RT-qPCR.
| Gene | Primer sequence (5ʹ – 3ʹ) |
|---|---|
| JAZF1 | F: ACGCCGAGAACAGGAAT |
| R: GTGCTGCTGCGGAATGAA | |
| Akt | F: AACGGACTTCGGGCTGTG |
| R: TTGTCCTCCAGCACCTCAGG | |
| VEGF | F: TGCACCCACGACAGAAGG |
| R: GCACACAG-GACGGCTTGA | |
| Bcl-2 | F: GCAACCGAACGCCCGCTGTG |
| R: GTGATGCAGGCCCCCACCAG | |
| Bax | F: AACAACATGGAGCTGCAGAGG |
| R: GAAGTTGCCGTCTGCAAACAT | |
| Caspase-3 | F: TACCCTGAAATGGGCTTGTGT |
| R: GTTAACACGAGTGAGGATGTG | |
| Caspase-9 | F: GGCCGGTGGACATTGGTTCTGG |
| R: CCATGAAGCGCAGCCAGCAGAA | |
| Bad | F: TGAACCGGCATCTGCACAC |
| R: TAGTGCACAGGGCCTTGA | |
| FKHR | F: AAGTTCTTGGTGGATGCTCAAT |
| R: GGGCGAAATGTACTCCAGTTAT | |
| PDGF | F:GCGGATACCTCGCCCATGCC |
| R:ACACTGCGGTGGTGGACCCT | |
| β-actin | F: ACCACAGCTGAGAGGGAAATCG |
| R: AGAGGTCTTTACGGATGTCAACG |
RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward; R, reverse; JAZF1, juxtaposed with another zinc finger protein 1; VEGF, vascular endothelial growth factor; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2 associated X protein; Bad, Bcl-2/Bcl-XL-associated death promoter; FKHR, Forkhead in rhabdomyosarcoma; PDGF, platelet-derived growth factor.