Figure 2.

Cellular distribution of choline at the mitotic exit. HeLa cells, synchronized by 2TB (a–d) or nocodazole (e–h), were incubated with 2 mM prop-Cho in complete media for 2 h (G₂/M to G₁ transition). The cells were fixed and reacted with 10 µM azide-Alexa488 (c and g). After counterstaining with Hoechst (b and f), the cells were imaged by fluorescence microscopy and phase contrast at 100X in an Olympus BX51 microscope. (a) and (e), bright field; (d) and (h), merged.