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Choline containing lipids synthesized as the cells transverse mitosis colocalize with ER membrane marker. HeLa cells, synchronized by 2TB and released for 8 h, were incubated with 2 mM prop-Cho for 2 h. The cells were fixed and processed for immunofluorescence with Alexa 488-azide (b) and Alexa 647-conjugated concanavalin A (c) and analyzed by confocal microscopy (Olympus FV1000 microscope). (a) Hoechst staining; (d) merged.
