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Choline containing lipids synthesized as the cells transverse mitosis colocalize with nuclear membrane marker. HeLa cells, synchronized by nocodazole, were incubated with 2 mM prop-Cho for 2 h. The cells were fixed and processed for immunofluorescence with Alexa 488-azide (a) and Cy3 conjugated anti-Mouse (for lamin A/C) (b) and analyzed by confocal microscopy (Olympus FV1000 microscope). (c) merged; (d) bright field.
