Figure 8.

Combined effect of etoposide and FASN impairment. Hela cells were treated with etoposide 80 μM for 24 h and the total cell number was estimated (a). The cells were incubated with etoposide and C75 at the concentrations shown in Table 1. The fraction affected and combination index were calculated as described under Methods (b). siRNA FASN or NT-transfected cells were incubated with etoposide 80 μM for 24 h and the FASN and cyclin B1 contents were assessed by Western Blot using vinculin as loading control.