Figure 1.

Delayed germination of raptor1b seeds.

A. raptor1b seeds have altered dormancy. Germination rate was scored from freshly harvested, non-stratified seeds. B. Germination of fully after-ripened WT and raptor1b seeds with or without stratification treatment. C. External application of nitrate did not improve the germination of raptor1b seeds. D. raptor1b seeds showed delayed germination with or without external supplementation of sucrose. Error bars indicate mean ± SD for 5 biological replicates. Black asterisks indicate significantly different from WT (P< .001, Student’s t-test). Stratification refers to seeds that have been kept for 3 days at 4°C. Values of germination percentages are from at least 5 biological replicates of 100 seeds each (100 seeds/plate/5 replicates).