Figure 4.

MR slows IS-induced senescence and suppresses the SASP in HK-2 cells. (a–c) Western blot analyses showing the levels of the senescence markers p53 and p21 in the three groups. (d) Serum-starved HK-2 cells were incubated with or without IS (250 μM) for 48 h; then, the cells were stained for SA β-gal, and the number of SA β-gal-positive cells was counted. (magnification ×400) (e and f) The levels of IL-1β and IL-6 in cell culture supernatants were analysed by ELISA. (g-i) Immunofluorescence results show IL-1β and IL-8 staining. The fluorescence intensity was lower in the IS+MR group than in the IS group. (magnification ×200). Three replicates were used for quantification. Values are presented as means ± SD. *P < 0.05, **P < 0.01.