Figure 6.
Suppression of IS-induced senescence in HK-2 cells by MR is regulated by H2S and AMPK/mTOR. Cells were incubated with 8 μM CC and with or without IS for 48 h. (a and b) Levels of the senescence markers p53 and p21 were analysed by Western blot. (c and d) Levels of IL-1β and IL-6 in cell culture supernatants were analysed by ELISA. (e) Cellular H2S concentrations. (f and g) Western blots showing changes in CGL and the AMPK/mTOR pathway. *p < 0.05，**p < 0.01，***p < 0.001 versus IS+MR. #p < 0.05 versus IS+MR.