Figure 2.

Primed defense responses of Arabidopsis aos and myc2 mutants.

Arabidopsis eight-rosette-leave plants were fed by second instar larvae of Spodoptera litura (Fabricius). (a) Primed plants and non-primed plants on the fourth day after infestation. (b) Rosette leaf damage measurement by ImageJ. (c) Larvae on the fourth day after infestation; the scale bar represents 5 mm. (d) Larval growth assessment after feeding on primed plants and non-primed plants. Ten larvae were used to calculate mean values and standard deviations. Feeding assay was replicated above 3 times. (e) Relative expression levels of VSP2 in primed plants and non-primed plants in wild type Col-0 and two mutants, aos and myc2. Error bars indicate the standard deviation. ** differences are highly significant (P< .01) by Dunnett’s multiple comparison test. dai: days after infection by larvae. NP: non-primed plants; P: primed plants.