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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Cell Metab. 2019 May 16;30(1):190–200.e6. doi: 10.1016/j.cmet.2019.04.013

Figure 1. The PPARGC1A 5′ UTR encodes an inhibitory uORF.

Figure 1.

(A) Ribosome profiling data indicating location of initiating (blue) or elongating (red) ribosomes on the PPARGC1A transcript in Jurkat cells. Initiating or elongating ribosomes were stalled using lactimidomycin or cycloheximide, respectively. Data adapted from (Gawron et al., 2016). (B) Ribosome profiling data indicating location of elongating ribosomes on the PPARGC1A transcript in HeLa cells. Data adapted from (Park et al., 2016; Wang et al., 2014; Zur et al., 2016). (C) Immunoblot (left) or RT– qPCR (right) of PGC1α protein or mRNA following transfection of each indicated expression vector in 293E cells (n=3). Dash indicates untransfected sample.