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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Cell Metab. 2019 May 16;30(1):190–200.e6. doi: 10.1016/j.cmet.2019.04.013

Figure 3. Inhibitory PPARGC1A uORFs are functionally conserved in fly and zebrafish but not in Atlantic bluefin tuna.

Figure 3.

(A) Top: schematic of GFP expression constructs that contain the mouse or fly PPARGC1A/spargel 5′ UTR. Bottom: Constructs were transfected into Drosophila SR2+ cells and GFP was monitored by microscopy. (B) Expression construct mRNA was injected into zebrafish zygotes and analyzed by fluorescence (images) or bioluminescence (graph). mCherry-Luc2 served as control for injection efficiency. N=20; p<0.01. (C) Alignment of conserved uORF within fish ppargc1a 5′ UTRs. The uORF start codon is highlighted in red, whereas the PGC1α start codon is highlighted in green. (D) Expression construct mRNA was injected into zebrafish zygotes and analyzed by fluorescence (images) or bioluminescence (graph). mCherry-Luc2 served as control for injection efficiency. N=20; p<0.01.