REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21(DE3)	Novagen	69450
Chemicals, Peptides, and Recombinant Proteins
Recombinant DNaseI (RNase free)	Roche	03539121103
T4 PNK	ThermoFisher	EK0031
Graphene Oxide	Sigma-Aldrich	763705-25ML
IPTG	ThermoFisher	R0392
Critical Commercial Assays
NEBNext Ultra II DNA Library Prep Kit for Illumina	NEB	E7645S
Deposited Data
Cas1Cas2Csn2 single complex bound to DNA	This work	PDB: 6QXF, EMD: 4668
Cas1Cas2Csn2 dimer complex bound to DNA	This work	PDB: 6QXT, EMD: 4670
Cas1Cas2Csn2 filament complex bound to DNA	This work	PDB: 6QY3, EMD: 4671
Oligonucleotides
acgaggagctattggcacaacttaca (prospacer duplex forward)	Macrogen	N/A
agttgtgccaatagctcctcgtcatt (prospacer duplex reverse)	Metabion	N/A
agatatcctacgaggttttagagctgtgttgtttcgaatggttccaaaacaaattctaaacgctaaagaggaag (CRISPR3 Leader-Repeat-Spacer duplex for in vitro integration reaction forward)	Metabion	N/A
cttcctctttagcgtttagaatttgttttggaaccattcgaaacaacacagctctaaaacctcgtaggatatct (CRISPR3 Leader-Repeat-Spacer duplex for in vitro integration reaction forward reverse)	Metabion	N/A
Recombinant DNA
pCRISPR3 (expressing full S. thermophilus CRISPR3 loci)	Sapranauskas et al., 2011	N/A
pCas9- (Cas9 deletion, containing the rest of the CRISPR3 loci)	Sapranauskas et al., 2011	N/A
pCas2- (Cas2 deletion, containing the rest of the CRISPR3 loci)	Sapranauskas et al., 2011	N/A
pCsn2- (Csn2 deletion, containing the rest of the CRISPR3 loci)	Sapranauskas et al., 2011	N/A
pCas1Strep (expressing C-Term Strep-tagged Cas1)	This work	N/A
pCR3ΔCRISPR (CRISPR region deletion, containing the rest of CRISPR3 loci)	This work	N/A
pCR3Δtracr (tracrRNA deletion, contain the rest of CRISPR3 locus)	Karvelis et al., 2013	N/A
Software and Algorithms
Phyre2 webserver	Kelley et al., 2015	http://www.sbg.bio.ic.ac.uk/∼phyre2/html/page.cgi?id=index
Chimera	Goddard et al., 2007	https://www.cgl.ucsf.edu/chimera/
COOT	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Refmac jelly-body refinement script	Murshudov et al., 1997	https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/em_fitting/em_fitting.html
PHENIX real space refine	Afonine et al., 2018	https://www.phenix-online.org/documentation/reference/real_space_refine.html
Motioncorr	Li et al., 2013	https://cryoem.ucsf.edu/software/driftcorr.html
Motioncor2	Zheng et al., 2017	https://msg.ucsf.edu/em/software/motioncor2.html
Gctf	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/
Gautomatch	N/A	https://www.mrc-lmb.cam.ac.uk/kzhang/
IMAGIC	van Heel and Keegstra, 1981	https://download.imagescience.de/index.html
RELION3	Zivanov et al., 2018	https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
cryoSPARC	Punjani et al., 2017	https://guide.cryosparc.com/install.html
GraphPad Prism 8	N/A	https://www.graphpad.com/scientific-software/prism/