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. 2019 Jul 10;8:e42271. doi: 10.7554/eLife.42271

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© 2019, Shen et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 9. — Since the fluorescent intensity of donor in control group was significantly lower, a subset of cells with comparable donor fluorescent intensity were selected as shown in red square for further analysis (top). FRET efficiency, mean fluorescent intensity of donor and mean fluorescent intensity of acceptor after matching the fluorescent intensity of control group and antigen group were shown (bottom).